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Phytochemical and biochemical composition of Azolla microphylla was evaluated. Petroleum ether, water, acetone, methanol, ethanol, chloroform, benzene, methylene chloride extracts were obtained. Phytochemical analysis of A.microphylla revealed the presence of alkaloids, terpenoids, steroids, coumarins, tannins, saponins, flavonoids, anthraquinones and phenols. Steroids and Tannins were present in most extracts while aromatic acids were absent in all the extracts. Dried and powdered leaves were estimated for protein, lipid, carbohydrate, ash and energy. Protein (0.252mg/g), Carbohydrate (0.178mg/g), Lipid (0.021mg/g) were present. Total ash 48%, acid-insoluble ash 50%, and water soluble ash 80% was also present.